The life-saving charity says rising aviation fuel prices could cost it an extra £45,000 a month.
The Court of Appeal has denied campaigners' efforts to challenge the closure of two bases.
When her husband died, Laura Davies thought she would never balloon again. now she is her own pilot.
Opponents are concerned relocating to a new base will delay helicopter response times.
Wales Air Ambulance boss says "a significant number" of people are missed in the evenings and at night.
Campaigners won the right to take their fight to the High Court, but a judge rejected their appeal.
Current plans would see two air ambulance stations permanently closed, with a new one opening.
The Prince of Wales spoke about Catherine's cancer treatment during a public appearance in Llanelli.
Both sites in Welshpool and Carmarthen will close, replaced by a new base in north Wales.
